Refeyn

“Unlike nsEM and cryo-EM, mass photometry allows us to look at our samples at the low nanomolar concentrations we use for activity assays... The MassFluidix HC was able to detect much longer filaments than expected – it turns out they tend to dissociate rapidly upon binding to the surface. We didn’t know our protein could do that!”

“Compared to SEC-MALS, mass photometry is fast and provides robust and reliable data… We also chose the MassFluidix HC as an add-on to the TwoMP, as some of our biophysical analyses do need to be conducted at higher concentrations.”

“I recently ran some manual, time-resolved samples on the Refeyn TwoMP, and the data was an absolute eye-opener. It generated clear, decisive insights that gave us a level of system understanding we simply couldn't have uncovered from SPR alone.”

“The underlying physics behind how it works are very complicated, but using the instrument is very much plug-and-play . We’ve got students in the lab using the instruments with no problems… One of the routine things that we are doing now is after solubilizing the protein and purifying it we put it into the mass photometer to check if there is a single peak of the right size before we then go on to do the expensive cryo-EM experiments.”

“The nice thing about mass photometry is that it happens in solution, without requiring labels or sample fixation . Therefore, I am sure of what to expect when I use magnetic tweezers, since I know how long my complex can remain stable at the desired temperature and conditions.”

“Mass photometry was the method of choice for our study, since it can quickly quantify the relative abundance of all the tau oligomers and show how these populations change over time , from the beginning of the aggregation process.”

“Mass photometry is very straightforward and convenient . You just need to dilute your sample in the right concentration and then you can quickly measure your sample in solution. You can also quickly analyze your measurements.”

“Our experience with the KaritroMP has been great - very easy, rapid quantification of empty/full particles in purified adenovirus samples with accuracy comparable to that of AUC .”

“[Our mass photometer] has been invaluable in a number of studies , ranging from simple cases of clarifying the oligomeric states of drug targets to elucidating complex binding mechanisms of small molecules.”

“The GMP software is simple, easy-to-use and all aspects of the workflow are captured in an audit trail. The automated analysis algorithm eliminates user bias and generates very repeatable data.”

“Mass photometry fits perfectly into our analytical workflows when we know there are additional species in our samples [such as antibody aggregates] but don't know their mass or oligomeric state. Now we finally have a suitable technology to fill that gap .”

“From the first moment I saw a mass photometer, I was mind-blown by how simple the instrument was to use and by how quickly you could see individual molecules and complexes... Since mass photometry requires very little sample, it is suitable for proteins that are hard to produce in large quantities... We also use mass photometry to quickly check our samples before using optical tweezers, to make sure we are really measuring single molecules.”

“Using mass photometry and the MassFluidix HC system gives us that control where we can track over time and see ‘now it’s this size’, ‘now it’s broken down to tetramers’, ‘now it’s dimers’. It’s more kinetic than we could be with any other technique.”

“It’s a game changer in AAV characterization, for us as well as our clients . In one project, for example, we saw that the partially filled AAV capsids were way more abundant than desired. Thanks to the SamuxMP, we could identify this rapidly and went back to the plasmid design... which led to drastically lower production of partials during plasmid expression, simplifying our downstream processing and purification workflows.”

“For any biochemical experiments that I will do in the future, I will always use mass photometry as a technique that either complements other techniques or replaces them entirely. I will try to keep mass photometry as integrated in my work as possible because it is a very honest technique that gives you a direct picture of your sample.”

“I’ve used AUC, A260/A280 and other techniques to measure adenovirus empty/full capsid ratios. The Karitro lets me do it quickly in the lab, with the accuracy of AUC.”